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Invitrogen™ LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit, goat

Brand:  Invitrogen™ PV4409

Additional Details : Weight : 0.00900kg

Product Code. 10302223

  • 17430.00 SEK / Pack of 800
Estimated Shipment: 16-04-2024
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For Research Use Only. All usage must comply with product instructions.


Description

Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15122:

The LanthaScreen™ TR-FRET Retinoic Acid Receptor (RAR) alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR alpha ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

How it works

The LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RAR alpha ligand-binding domain (RAR alpha-LBD) that is tagged with glutathione-S-transferase (GST). To assay, RAR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit contains RAR alpha-LBD (GST) protein, fluorescein-labeled D22 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications

Specifications

Tb (Terbium)
Microplate Reader
Store in ultra-cold freezer (-68 to -85°C).
5914
Dry Ice
LanthaScreen™
RAR alpha
Fluorescent
384-well Plate
Biochemical coregulator interaction
End Point
RARA, RAR alpha, NR1B1
Co-Factor Interaction Assay, TR-FRET
TR-FRET RAR Alpha Coactivator Assay Kit
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For Research Use Only. Not for use in diagnostic procedures.