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Ly-6C, APC-eFluor 780, clone: HK1.4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-5932-82

Additional Details : Weight : 0.01000kg

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Product Code. 15261732

  • 2490.00 SEK / 100µg


EU Stock 6
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Description

Description

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Ly-6C
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
P0CW02
AA682074, AA959465, Ly-6C, Ly6c, Ly6c2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
HK1.4
APC-eFluor 780
Ly6c1
Conjugated
Ly6c1
IgG2c, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
17067
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