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Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor™ 780, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

1312.00 SEK - 3235.00 SEK

Specifications

Antigen Ly-6C
Clone HK1.4
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15539596
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Affymetrix eBioscience
47-5932-80
25 μg
1312.00 SEK
25µg
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15261732
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Affymetrix eBioscience
47-5932-82
100 μg
3235.00 SEK
100µg
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Description

Description

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells. In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release. Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Ly-6C Monoclonal antibody specifically detects Ly-6C in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

Ly-6C
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
AA682074; AA959465; Ly6c; Ly-6C; Ly6c1; Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C
Ly6c1
Primary
4° C, store in dark, DO NOT FREEZE!
Ly6c1
HK1.4
Flow Cytometry
APC-eFluor 780
Rat
Mouse
P0CW02
17067
IgG2c κ
Affinity chromatography
Antibody
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