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Ly-6C Monoclonal Antibody (HK1.4), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

1256.00 SEK - 2995.00 SEK

Specifications

Antigen Ly-6C
Clone HK1.4
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15298919
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Affymetrix eBioscience
12-5932-80
25 μg
1256.00 SEK
25µg
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15208929
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Affymetrix eBioscience
12-5932-82
100 μg
2995.00 SEK
100µg
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Description

Description

Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells. In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release. Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
Specifications

Specifications

Ly-6C
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
AA682074; AA959465; Ly6c; Ly-6C; Ly6c1; Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C
Ly6c1
Primary
4° C, store in dark, DO NOT FREEZE!
Ly6c1
HK1.4
Flow Cytometry
PE
Rat
Mouse
P0CW02
17067
IgG2c κ
Affinity chromatography
Antibody
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