Ly-6G, Functional Grade, Clone: 1A8-Ly6g, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-9668-82
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown.Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity.Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis and depletion of neutrophils. Moreover, this antibody has been reported to have antagonist activity. (Fluorochrome-conjugated 1A8-Ly6g is recommended for use in flow cytometry.).Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Gr-1, Gr1, Ly-6G|
|Flow Cytometry, Functional Assay|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.