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Invitrogen™ LYVE-1 Human Uncoated ELISA Kit with plates
Uncoated ELISA Kit with Plates
Brand: Invitrogen™ 88-52239-22
Additional Details : Weight : 0.30000kg
Description
The Human LYVE-1 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human LYVE-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Specifications
Q9Y5Y7 | |
1.09 pg/ml | |
ELISA Kit | |
Human | |
10894 | |
Capture Antibody, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Chromagen, 96-well Microtiter Plates (2) | |
2 x 96 Tests | |
Serum, 50 μL; Plasma, 50 μL; Cell Lysate,50 μL; Supernatant, 50 μL | |
Human | |
3 hr. 15 min. |
1.09-70 pg/ml | |
Biotin | |
Cell Lysates, Plasma, Serum, Supernatant | |
Colorimetric Microplate Reader | |
LYVE1 | |
HRP | |
RUO | |
2°C to 8°C | |
1 hr. 20 min. |