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Beskrivning
Thermo Scientific MagnaBind Streptavidin Beads are convenient for affinity purification or separation methods involving of biotin-labeled molecules. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.
Features of MagnaBind Streptavidin Beads:
- Composition: Silanized iron oxide
- Magnetization: 25-35EMU/g
- Type of Magnetization: Superparamagnetic (no magnetic memory)
- Surface Area: >100m2/g
- Bead Size: 1-4μm diameter
- Settling Rate: 4% in 30 minutes
- Effective Density: 2.5g/mL
- Number of Beads: 1 x 108 beads/mg
- pH Stability: Aqueous solution, above pH 4.0
- Concentration: 5 mg/mL
Streptavidin is a 60kDa protein from Streptomycetes avidinii. The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.
Applications:
- Cell sorting using either positive or negative selection
- Protein purification or immunoassays using either direct or indirect methods
Specifikationer
Specifikationer
| Koncentration | Slurry: 5 mg/mL |
| Innehåll och lagring | Vid mottagandet lagra produkten vid 4 °C. Frys inte produkten. Produkten levereras vid rumstemperatur. |
| Beskrivning | MagnaBind Streptavidin pärlor |
| Färg | Brun |
| För användning med (applikation) | Affinitetsrening |
| Produkttyp | Streptavidin Magnetic Beads |
| Ligandtyp | Streptomycin |
| Produktlinje | MagnaBind |
| Kvantitet | 5 ml |
| Volym (metrisk) | 5 mL |
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Endast för forskning. Ej för diagnostik.
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