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MAP4K3 (GLK) Recombinant Human Protein

MAP4K3 (GLK) Recombinant Human Protein

Brand:  Invitrogen PV6351

UNSPSC : 12352202

Additional Details : Weight : 0.01000kg

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Product Code. 15478983

  • 45530.00 SEK / 1mg


Estimated Shipment date
from Supplier 01-09-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

MAP4K3 (also known as GLK) is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. MAP4K3 is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-alpha and can lead to the activation of the JNK signaling pathway. MAP4K3 can directly activate PKC-Θ, during TCR signaling and people with systemic lupus erythematosus show considerable enhanced MAP4K3 expression and activation of PKC-Θ, and the kinase IKK in T cells. MAP4K3 can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM. Recombinant human MAP4K3 (1-380) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

  • Validated in LanthaScreen™ Kinase Binding Assay
  • Protocols available for simple use and optimized performance
  • Multiple pack sizes available for convenient use

    Related Product:
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  • Specifications

    Specifications

    NP_003609.2
    Pharma and biopharma, protein assays and analysis, target and lead identification and validation
    MAP4K3 (GLK) Recombinant Human Protein
    1mg
    For research use only. Not for use in diagnostic procedures
    MAP4K3, MAPKKKK3, MEKKK3, RAB8IPL1
    MAP4K3 (GLK) was pre-diluted in enzyme dilution buffer and assayed in 5mM MOPS (pH 7.2), 2.5mM β-glycerol-phosphate, 5mM MgCl2, 0.4mM EDTA, 1mM EGTA, 0.05mM DTT, with 50μM ATP, trace [33P]-γ-ATP and 200μg/mL PKA Substrate peptide (CGRTGRRNSI-amide) for 15 min.s at 30°C
    Amino acids 1-380
    84nmoles of phosphate transferred to PKA Substrate peptide (CGRTGRRNSI-amide) per minute per mg of total protein at 30°C
    GST Tag
    0.05mg/mL
    69.6kDa
    >70% by SDS-PAGE
    –68 to –85°C
    1 Each
    MAP4K3 (GLK) Recombinant Human Protein
    Kinase assay
    MAP4K3 (GLK) Recombinant Human Protein
    Human