Mature Macrophage Marker, clone: eBio25F9 (25F9), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0115-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other hematopoietic cell.Applications Reported: This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen and paraffin embedded tissue sections.Applications Tested: This eBio25F9 (25F9) antibody has been tested by flow cytometric analysis of human macrophages. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Mature Macrophage Marker|
|PBS with 0.09% sodium azide; pH 7.2|
|ED2-like antigen, Mature Macrophages, Macrophages|
|Store at 2-8°C.|
|Flow CytometryþImmunohistochemistry (Frozen)þImmunohistochemistry (Paraffin)þImmunoprecipitationþWestern Blot|
For Research Use Only.