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MCL-1, clone: LVUBKM, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9047-82

Additional Details : Weight : 0.09500kg

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Product Code. 15507576

  • 2530.00 SEK / 100µg


Estimated Shipment date
from Supplier 24-07-2018
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Description

Description

Description: This LVUBKM monoclonal antibody recognizes human and mouse myeloid cell leukemia sequence 1 (Mcl-1). Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins important for regulation of cell survival/apoptosis. Mcl-1 is primarily localized to the outer membrane of mitochondria where it prevents cytochrome c release via dimerization with other Bcl-2 family members such as Bim. Although it is expressed in both immune and non-immune cells, highest levels of Mcl-1 expression are seen in hematopoietic lineage cells. PI3K activation of AKT results in destabilization and degradation of GSK3 beta, which prevents phosphorylation of Mcl-1 on S159 and its subsequent ubiquitnation and degradation. Mice conditionally lacking Mcl-1 in lymphocytes showed that Mcl-1 is essential during early lymphoid development and for the maintenance of mature lymphocytes.Applications Reported: This LVUBKM antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and intracellular staining followed by flow cytometric analysis. (Fluorochrome-conjugated LVUBKM is recommended for use in intracellular flow cytometry.).Applications Tested: This LVUBKM antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Mcl-1
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P97287, Q07820
bcl-2-like protein 3, bcl-2-related protein EAT/mcl1, EAT, MCL1L, MCL1S, MGC104264, MGC1839, myeloid cell leukemia 1, myeloid cell leukemia ES, myeloid cell leukemia sequence 1 (BCL2-related)
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
Flow CytometryþImmunohistochemistryþImmunohistochemistry
LVUBKM
Unconjugated
MCL1
Purified
Mcl1
IgG1, kappa
100μg
Store at 2-8°C.
Primary
4170
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