MD-2, clone: 9B4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9928-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 9B4 antibody reacts with human MD-2, an approximately 30kDa molecule, which physically associates with the extracellular domain of TLR4. MD-2 is essential for correct intracellular distribution and LPS-recognition of TLR4 since in MD-2(-/-) embryonic fibroblasts, TLR4 was not able to reach the plasma membrane and predominantly resided in the Golgi apparatus, whereas TLR4 was distributed at the leading edge surface of cells in wild-type embryonic fibroblasts. MD-2(-/-) mice do not respond to LPS, do survive endotoxic shock but are susceptible to Salmonella typhimurium infection.Applications Reported: The 9B4 antibody has been reported for use in immunoprecipitation.Applications Tested: This 9B4 antibody has been tested by immunoprecipitation of recombinant MD-2 from cell supernatant. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Store at 2-8°C.|
For Research Use Only.