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MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 46-5958-80
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Beskrivning
Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed. Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
Specifikationer
| MHC Class I (H-2Kb) | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
| P01902 | |
| H2-K1 | |
| Affinity chromatography | |
| RUO | |
| 14972 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| AF6-88.5.5.3 | |
| PerCP-eFluor 710 | |
| H2-K1 | |
| H-2K, H-2K(d), H2-K, K-f,H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC H2-K transplantation antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I antigen; MHC class I heavy chain H2-K; class I major histocom | |
| Mouse | |
| 25 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2a κ |
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Produkttitel
For Research Use Only.
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