MHC Class I (H-2Kd/H-2Dd) Mouse anti-Mouse, eFluor® 450, Clone: 34-1-2S, eBioscience™

Description

Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p. Applications Reported: This 34-1-2S antibody has been reported for use in flow cytometric analysis. Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.