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MHC Class I (H-2Kd/H-2Dd), PerCP-eFluor™ 710, clone: 34-1-2S, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5998-80

Additional Details : Weight : 0.09500kg

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Product Code. 15509336

  • 790.00 SEK / 25µg


Estimated Shipment date
from Supplier 27-08-2018
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Description

Description

Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p.Applications Reported: This 34-1-2S antibody has been reported for use in flow cytometric analysis.Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

MHC Class I (H-2Kd/H-2Dd)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01902, P14426
H-2 class I histocompatibility antigen, K-D alpha chain, H2K1, H2KD, H2DD
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
34-1-2S
PerCP-eFluor 710
H2-D1
Conjugated
H2-D1, H2-K1
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
14964, 14972
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