MHC Class I (H-2Kd/H-2Dd), PerCP-eFluor™ 710, clone: 34-1-2S, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5998-80
Additional Details : Weight : 0.09500kg
DescriptionDescription: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p.Applications Reported: This 34-1-2S antibody has been reported for use in flow cytometric analysis.Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|MHC Class I (H-2Kd/H-2Dd)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|H-2 class I histocompatibility antigen, K-D alpha chain, H2K1, H2KD, H2DD|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.