MHC Class II (I-Ek), PE, clone: 14-4-4S, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5980-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat.Applications Reported: 14-4-4S has been reported for use in flow cytometric analysis.Applications Tested: The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|MHC class II (I-Ek)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|AI323765, E-alpha-f, H-2Ea, I-Ealpha, Ia-3, Ia3, MGC117888|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.