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IBA Lifesciences™ MHC I-Strep H-2 Db; gp33 LCMV (KAVYNFATM); Fused to Twin-Strep-tag™

MHC Class I molecule fused to a streptavidin derivative and refolded with a peptide.

Brand:  IBA Lifesciences™ 6-7016-001

Additional Details : Weight : 0.00200kg

Product Code. 17540635

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Description

Description

MHC I-Strep H-2 Db; gp33 LCMV (KAVYNFATM) is a recombinantly expressed MHC Class I molecule that presents a peptide from a lymphocytic choreomeningitis virus (LCMV) glycoprotein 33 (gp33). Our MHC I Streptamer™ approach is based on our Strep-tag™ technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag™ fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin™ backbone, resulting in the formation of MHC I Streptamers.

For multimerizing MHC I-Streps, Strep-Tactin™ TACS Agarose columns, Strep-Tactin™ Magnetic Microbeads or fluorophore-conjugated Strep-Tactin™ can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements.

  • Addition of biotin reverses multimerization of MHC Class I molecules, leading to dissociation from target cells
  • Resulting label-free cells are highly viable and fully functional
Specifications

Specifications

Dissolved in DPBS
10 μg
Human
Cell Isolation
Antigen-specific T Cell
0.2 μg per 2 x 106 Total Cells
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