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IBA GmbH™ MHC I-Strep HLA-A*0201 CMV IE-1 VLEETSVML

IBA GmbH™ MHC I-Strep HLA-A*0201 CMV IE-1 VLEETSVML is for fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells.

Brand:  IBA GmbH™ 6-7041-001

Additional Details : Weight : 0.00010kg

Product Code. 15693918

  • 4262.00 SEK / 40µL


Estimated Shipment
03-10-2019
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Description

Description

  • Peptide (antigen)-loaded MHC I-Streps (Strep-tagged MHC I molecules) are multimerized with Strep-Tactin™ attached to a fluorochrome to generate multimers with high binding avidity. These multimers are determined for staining of T cells which are specific for the respective peptide-loaded MHC molecule
  • After FACS analysis or FACS sorting of the target cells, the staining reagents can be completely removed with biotin under physiological conditions at low temperatures (4°C). The monomeric low affinity MHC I-Strep molecules are then dissociated completely from the cell surface by two short washing steps. Target cells are left phenotypically and functionally indistinguishable from untreated cells.
  • Form: Dissolved in DPBS (Dulbecco's phosphate buffered saline) without Ca and Mg, contains KCl; pH7.9 - 8.1
  • Solution is sterile and does not contain any preservatives
  • For fluorescent staining of 5 x 107 to 1 x 108 cells
  • For magnetic isolation out of 1 x 108 cells
  • Long-term storage: -80°C (avoid repeated freezing and thawing)
  • Short-term storage: make aliquots and store at 2 to 8°C
  • Product is not classified as hazardous according to (EC) No 1272/2008 [CLP]
Specifications

Specifications

40μL
Fluorescent reagent for staining, magnetic T cell labeling and isolation
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