MICA/B, clone: 6D4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5788-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. (Please use Functional Grade purified 6D4, cat. 16-5788, in functional assays.).Applications Tested: This 6D4 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|MHC class I polypeptide-related sequence A, MHC class I polypeptide-related sequence B|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.