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Molecular Probes™ MitoProbe™ JC-1 Assay Kit

MitoProbe™ JC-1 Assay Kit

Brand:  Molecular Probes™ M34152

Additional Details : Weight : 0.11000kg

Product Code. 10226762

  • 6390.00 SEK / Each


EU Stock 12
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For Research Use Only. All usage must comply with product instructions.


Description

Description

The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry.

View a selection guide for all apoptosis assays for flow cytometry.

Selective to mitochondrial changes
The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~529 nm) to red (~590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates.

Unlike other mitochondrial stains that are affected by plasma membrane potential, the ratio of green to red fluorescence of JC-1 depends only on the membrane potential and not other factors such as mitochondrial size, shape, and density, which may influence single-component fluorescence signals. Use of fluorescence ratio detection allows researchers to make comparative measurements of membrane potential and determine the percentage of mitochondria within a population that respond to an applied stimulus.

Indicator of early apoptosis
A distinctive feature of the early stages of programmed cell death is the disruption of active mitochondria. This mitochondrial disruption includes changes in the membrane potential and alterations to the oxidation-reduction potential of the mitochondria. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibration of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol.

Simple, streamlined protocol
One of the biggest challenges to working with dual-emitting dyes like JC-1 is compensation. The MitoProbe™ JC-1 Assay Kit provides a mitochondrial membrane-potential disrupter, CCCP, in order to produce compensation controls leading to a correctly compensated green-to-red fluorescence ratio. The stain protocol is simple and straightforward: incubate the cells with JC-1 dye and then analyze the fluorescent signal in the FITC and PE channels of of a 488 nm laser.
Specifications

Specifications

MitoProbe™ JC-1 Assay Kit
5 vials of JC-1 (5A€², 6, 6A€²-tetrachloro-1, 1A€², 3, 3A€²-tetraethylbenzimidazolylcarbocyanine iodide) (30μg/vial of powdered dye), 1 vial of DMSO (1.5mL), 1 vial of CCCP (125μL, 50mM in DMSO), and 1 bottle of 10X phosphate-buffered saline (25mL)
Cell viability, proliferation and function, cellular Imaging, flow cytometry
Solid
MitoProbe™ JC-1 Assay Kit, 1 Kit, 100 rxns, Solid, Red/Orange, 652.23 Molecular Weight, C25H27Cl4IN4 Molecular Formula, 590nm Emission, 514/529 Excitation, 488 Flow Cytometer Laser Lines, Low Water Solubility, 2 to 8°C Storage Temperature, Room
2 to 8°C
Orange
590nm
Flow cytometer
5 vials of JC-1 (5A€², 6, 6A€²-tetrachloro-1, 1A€², 3, 3A€²-tetraethylbenzimidazolylcarbocyanine iodide) (30μg/vial of powdered dye), 1 vial of DMSO (1.5mL), 1 vial of CCCP (125μL, 50mM in DMSO), and 1 bottle of 10X phosphate-buffered saline (25mL)
100 rxn(s)
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For Research Use Only. Not for use in diagnostic procedures.