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Invitrogen™ Human MMP3 Matched Antibody Pair

Produktkod. 10184603
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Kvantitet:
10 x 96 Tests
Förpackningsstorlek:
Varje
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10184603 10 x 96 Tests Varje
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For Research Use Only. All usage must comply with product instructions.
Produktkod. 10184603 Leverantör Invitrogen™ Leverantörsnummer CHC1543
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Beräknad leverans: 13-08-2026
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For Research Use Only. All usage must comply with product instructions.

Beskrivning

ELISA

The Human MMP3 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MMP3. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.
TRUSTED_SUSTAINABILITY

Specifikationer

Tillträdesnummer P08254
Analysintervall 1.25-20 ng/mL
Analyskänslighet <1.25 ng/mL
Konjugera HRP
Genaccessionsnr. P08254
Gene Alias CHDS6, MGC126102, MGC126103, MGC126104, MMP-3, SL-1, STMY, STMY1, STR1, CHDS6; EC 3.4.24.17; EMS-2; matrix metallo protease; matrix metallopeptidase 3; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 precursor; matrix metalloproteinase-3; metalloproteinase 3 receptor; MMP; MMP10; MMP3; MMP-3; MMP-3 protein; MMPs; progelatinase; proteoglycanase; SL-1; SLN1; SLN-1; STMY; STMY1; Str1; STR-1; stromelysin; stromelysin 1; Stromelysin1; stromelysin-1; Transin1; Transin-1CHDS6; EC 3.4.24.17; EMS-2; matrix metallo protease; matrix metallopeptidase 3; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 precursor; matrix metalloproteinase-3; metalloproteinase 3 receptor; MMP; MMP10; MMP3; MMP-3; MMP-3 protein; MMPs; progelatinase; proteoglycanase; SL-1; SLN1; SLN-1; STMY; STMY1; Str1; STR-1; stromelysin; stromelysin 1; Stromelysin1; stromelysin-1; Transin1; Transin-1; CHDS6; MMP-3; SL-1; STMY; STMY1; STR1
Gensymboler MMP3
Immunoassay Kit Format Matched Antibody Pair
Produkttyp ELISA, ELISA Kit
Kvantitet 10 x 96 Tests
Provtyp Plasma, Serum, Supernatant
Regulatorisk status RUO
Typ av substrat Proteases
Gen-ID (Entrez) 4314
Målarter Human
För användning med (utrustning) Absorbance Microplate Reader, Colorimetric Microplate Reader
Inkluderar Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Innehåll och lagring Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gensymbol MMP3
Kit Innehåll Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Etikett eller färgämne Biotin
Proteinaliaser SL-1, Matrix metalloproteinase-3, MMP-3, Transin-1
Frakt skick Wet Ice
Förvaringskrav 2-8°C
Mål MMP3
Testtid 1 hr 30 min
Total analystid 24 hr
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