Non-radioactive technique
Brand: Invitrogen™ C10102
Click-iT AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or 'click' reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT Protein Analysis Detection Kits containing either TAMRA, Dapoxyl™, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS/MS and MALDI MS analysis or Multiplexed Proteomics™ reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein.
Cell Analysis, Click Chemistries, Labeling Chemistry, Metabolic Labeling and Nascent Protein Synthesis with Click Chemistry, Protein and Antibody Labeling, Proteins, Expression, Isolation and Analysis
Shipping Condition: Room temperature
| Less hazardous | |
| 5 mg | |
| Biotin-based, Fluorescence | |
| Room Temperature | |
| Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine) |
| AHA | |
| Store in freezer -5°C to -30°C. | |
| Metabolic Labeling | |
| Click-iT™, Molecular Probes™ | |
| Proteins |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Expires: 2025-05-31
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.
