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Invitrogen™ Molecular Probes™ Rhod dextran, Potassium Salt, 10,000 MW, Anionic (High-Affinity Version)
Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells
Brand: Invitrogen™ R34676
Additional Details : Weight : 0.00525kg
Description
Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.
- Rhod coupled to 10,000 MW dextran can be dissolved in water at concentrations up to 20% w/v (200μg/uL) and introduced via patch pipet, microinjection, or Influx™ pinocyte-loading reagent
- Fluorescence signal from these cells is generally measured using fluorescence microscopy
- Dextran forms of calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms
- Label (Ex/Em of Ca2+-bound form): Rhod (552/581nm)
- Fluorescence intensity increase upon binding Ca2+: >100 fold
- Kd for Ca2+ in the absence of Mg2+, in buffer: approximately 750nM
- Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
- In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
- Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN
Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling
Order Info
Shipping Condition: Room temperature
Specifications
| Store in freezer (-5° to -30°C) and protect from light. | |
| Fluorescence Microscope |
For Research Use Only. Not for use in diagnostic procedures.