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Invitrogen™ SBFI, Tetraammonium Salt, cell impermeant

Sodium-sensitive molecule used to estimate Na+ gradients in isolated mitochondria, to measure intracellular NA+ levels, or to measure Na+ efflux in cells

Brand:  Invitrogen™ S1262

Product Code. 10033152

  • 8200.00 SEK / 1mg

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

  • Useful in combination with other fluorescent indicators used to correlate changes in intracellular Na+ with Ca2+ and Mg2+ concentrations, intracellular pH, and membrane potential
  • Although selectivity of SBFI for Na+ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na+ in presence of other monovalent cations
  • The spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-2
  • Label (Ex/Em): SBFI (approximately 340,380/500nm)
  • Lyophilized product may be dissolved in distilled water or aqueous buffer for use
  • Product is typically loaded into cells by diffusion from patch pipet for correlated fluorescence imaging and electrophysiological recording
  • Dissociation constant (Kd) of SBFI for Na+ is 3.8mm in the absence of K+, and 11.3mm in solutions with combined Na+ and K+ concentration of 135mm (which approximates physiological ionic strength). SBFI is approximately 18-fold more selective for Na+ than for K+

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store at room temperature and protect from light.
Fluorescence Microscope
Sodium Indicator
Room Temperature
Fluorescence
SBFI Tetraammonium Salt
1 mg
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For Research Use Only. Not for use in diagnostic procedures.