eBioscience™ SAFE™ Human IL-4, Recombinant Protein
Human Recombinant Protein
Brand: Affymetrix eBioscience 68-8780-63
Additional Details : Weight : 0.09500kg
DescriptionThis SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.
Human IL-4 is also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.
|17.2kDa (apparent 23 and 24kDa)|
|The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile low salt buffer to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.|
|Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. Accession number NP_000580.|
|The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.|
|Sterile lyophilized (PBS buffer pH 7.2)|
|Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay|