Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™, Invitrogen™

Description

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis. Applications Tested: The APC conjugated mouse IgG2a isotype control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at less than or equal to 1 µg/million cells (100 µL blood). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at test size: 5 µL (1 µg)/test per million cells (100 µL blood). Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.