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Mouse CD254 (RANK Ligand), Invitrogen™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8612-80

Additional Details : Weight : 0.09500kg

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Product Code. 15567546

  • 3020.00 SEK / 25µg

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This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. Description: RANK Ligand (Receptor Activator for Nuclear Factor kappaB Ligand), also known as TNF-related activation-induced cytokine (TRANCE), along with RANK are members of the TNF superfamily. RANK-L can be found as a soluble protein expressed by activated T lymphocytes, which plays a role in dendritic cell maturation. In addition it has also been shown to be cleaved by TACE (TNF-alpha convertase) releasing an ectodomain variant of the cell bound form. Lastly RANK-L is expressed as a Type II transmembrane protein expressed by osteoblast lineages. The receptor RANK, located on osteoclastic and dendritic cells can recognize all forms of RANK-L. RANK-L plays a critical role in bone development and mineralization. High levels of RANK-L have been associated with degenerative bone diseases. Applications Reported: Recombinant mouse RANK-L is biologically active. Applications Tested: ED50, as measured by induction of osteoclast formation on mouse RAW264.7 cells, is 4.8ng/mL. Source: E. coli expressed amino acid Lys158-Asp316 accession # Q3TWY5. Bioactivity: The ED50, as measured by induction of osteoclast formation on mouse RAW264.7 cells, is 4.8ng/mL. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE. Molecular Weight: 20 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.


Control, Functional Assay
Control, Functional Assay
PBS with 1% BSA and no preservative; pH 7.2
Affinity chromatography
E. coli
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Recombinant Protein
E. coli
Mouse CD254 (RANK Ligand)
reducing SDS-PAGE
E. coli expressed amino acid Lys158 - Asp316 Accession # Q3TWY5
Mouse CD254 (RANK Ligand)
The ED50, as measured by induction of osteoclast formation on mouse RAW264.7 cells, is 4.8ng/mL.