Invitrogen™ eBioscience™ Mouse IL-15/IL-15R Platinum ELISA Kit
For the quantitative detection of mouse IL-15/IL-15R.
Brand: Invitrogen™ BMS6023TEN
Additional Details : Weight : 0.23750kg
DescriptionInterleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
|Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers|
|10 x 96 tests|
|1 hr 20 min|
|Interleukin 15, Interleukin 15 Receptor|
|Colorimetric Microplate Reader|
|Plasma, Serum, Supernatants|
|Plasma (50μL), Serum (50μL), Supernatants (50μL)|
|3 hr 30 min|
For Research Use Only