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Invitrogen™ eBioscience™ Mouse IL-15/IL-15R Platinum ELISA Kit

For the quantitative detection of mouse IL-15/IL-15R.

Brand:  Invitrogen™ BMS6023TEN

Additional Details : Weight : 0.23750kg

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Product Code. 15501997

  • 21860.00 SEK / Pack of 10


Estimated Shipment date
from Supplier 02-07-2019
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-15/IL-15R
Biotin-Conjugate anti-mouse IL-15/IL-15R polyclonal antibody
Streptavidin-HRP
Mouse IL-15/IL-15R Standard lyophilized, 400pg/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Sample Diluent
Calibrator Diluent
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
Specifications

Specifications

P48346, Q60819
Biotin
16168, 16169
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
10 x 96 tests
2.0 pg/mL
50μL
Mouse
1 hr 20 min
Mouse
Interleukin 15, Interleukin 15 Receptor
5.3%
6.3-400 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Plasma (50μL), Serum (50μL), Supernatants (50μL)
2-8°C
3 hr 30 min
Il15, Il15ra
6.3%
HRP
Videos

For Research Use Only