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Invitrogen™ IL-18 Mouse ELISA Kit

For the quantitative detection of mouse IL-18.

6060.00 SEK - 32360.00 SEK

Specifications

Accession Number P70380
Assay Range 31.3-2,000 pg/mL
Assay Sensitivity 19.0 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-18
Biotin-Conjugate anti-mouse IL-18 monoclonal antibody
Streptavidin-HRP
Mouse IL-18 Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

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Product Code Brand Quantity Price Quantity & Availability  
15552047
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Invitrogen™
BMS618-3
96 Tests
6060.00 SEK
96 tests
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15562047
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Invitrogen™
BMS618-3TEN
10 x 96 Tests
32360.00 SEK
Pack of 10
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Estimated Shipment: 10-04-2024
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Description

Description

The Mouse Interleukin-18 (Ms IL-18) ELISA quantitates Ms IL-18 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-18. Principle of the method The Mouse IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency
Specifications

Specifications

P70380
19.0 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
Igif,Il-18
2.2%
HRP
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Interleukin-18, Interferon-gamma-inducing factor
1 hr. 20 min.
31.3-2,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
16173
2.2%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Mouse
3 hr. 10 min.
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