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Invitrogen™ IL-1 beta Mouse ELISA Kit

For the quantitative detection of mouse IL-1beta.

6070.00 SEK - 32360.00 SEK

Specifications

Accession Number P10749
Assay Range 7.8-500 pg/mL
Assay Sensitivity 1.2 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-1beta
Biotin-Conjugate anti-mouse IL-1beta monoclonal antibody
Streptavidin-HRP
Mouse IL-1beta Standard lyophilized, 1ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

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15571957
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Invitrogen™
BMS6002
96 Tests
6070.00 SEK
96 tests
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Invitrogen™
BMS6002TWO
2 x 96 Tests This item is currently unavailable or has been discontinued.
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Invitrogen™
BMS6002TEN
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Description

Description

The Mouse Interleukin-1beta (Ms IL-1β) ELISA quantitates Ms IL-1β in mouse serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-1β. Principle of the method The Mouse IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

IL-1beta is a pro-inflamatory cytokine and is expressed by many cells including macrophage, NK cells, monocytes, and neutrophils.
Specifications

Specifications

P10749
1.2 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
IL-1beta,Il-1b
4.7%
HRP
Serum, 50 μL; Supernatant, 50 μL
Catabolin, Interleukin-1 beta, IL1F2
1 hr. 20 min.
7.8-500 pg/mL
Biotin
Serum, Supernatant
ELISA
16176
5.7%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Mouse
3 hr. 10 min.
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