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Mouse TNF alpha Carrier-Free, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 34-8321-85

Additional Details : Weight : 0.09500kg

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Product Code. 15518786

  • 13450.00 SEK / 500µg


Estimated Shipment date
from Supplier 24-08-2018
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Description

Description

Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single ∽17kDa band following SDS-PAGE under non-reducing conditions. Description: Mouse TNF alpha is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed. Applications Reported: Recombinant mouse TNF alpha is biologically active and can induce cytolysis of the L929 mouse cell line in culture. Applications Tested: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF alpha accession # NM_013693. Bioactivity: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Specifications

Specifications

P06804
Cytokine Bioassays
PBS with no preservative; pH 7.2
The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
7.2
Affinity chromatography
E. coli
RUO
Recombinant
Mouse TNF alpha Carrier-Free
Control, Functional Assay
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
0.5mg/mL
Liquid
21926
Mouse TNF alpha Carrier-Free
SDS-PAGE
500μg
-80°C
E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF alpha accession # NM_013693
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Tnf
The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg.
Mouse
Ms
Recombinant
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