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Invitrogen™ IL-21 Mouse ELISA Kit

For the quantitative detection of mouse IL-21.

Brand:  Invitrogen™ BMS6021TEN

Additional Details : Weight : 0.23750kg

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Product Code. 15561987

  • 22630.00 SEK / Pack of 10


Estimated Shipment
09-12-2020
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-21
Biotin-Conjugate anti-mouse IL-21 monoclonal antibody
Streptavidin-HRP
Mouse IL-21 Standard lyophilized, 2000pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.
Specifications

Specifications

Q9ES17
Biotin
60505
ELISA Kit
5.0 pg/mL
Plasma, 50 μL|Serum, 50 μL|Supernatant, 50 μL
1 hr. 20 min.
Mouse
Za11, Interleukin-21, Cytokine CX2
5.2%
Mouse
HRP
16.1-1,000 pg/mL
ELISA
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
50 μL
2°C to 8°C
3 hr. 30 min.
IL-21
5.1%
10 x 96 Tests
Supernatant
Colorimetric Microplate Reader
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For Research Use Only