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Invitrogen™ IL-22 Mouse Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of mouse IL-22 protein levels from samples including serum, and cell culture supernatants
2305.00 SEK - 5230.00 SEK
Specifications
| Accession Number | Q9JJY9 |
|---|---|
| Assay Range | 8-1,000 pg/mL |
| Assay Sensitivity | 8 pg/mL |
| Conjugate | Biotin |
| Product Type | ELISA Kit |
| Product Code | Brand | Quantity | Gene Symbol | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Gene Symbol | Price | Quantity & Availability | ||||
|
15561147
|
Invitrogen™
88-7422-86 |
10 x 96 Tests | GMCSF |
5230.00 SEK
Pack of 10 |
Estimated Shipment: 27-05-2024
Log in to see stock. |
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|
15551147
|
Invitrogen™
88-7422-22 |
2 x 96 Tests | GMCSF |
2305.00 SEK
Pack of 2 |
Estimated Shipment: 10-05-2024
Log in to see stock. |
||||
Description
The Mouse Interleukin 22 (IL-22) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-22. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
IL-22 Mouse Uncoated ELISA Kit (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)- The assay demonstrates parallelism in measuring recombinant and native mouse IL-22 proteins and a standard curve range of 8 to 1000pg/mL
- Human IL-22 receptor at levels above 4ng/ml and IL-22 binding protein at level above 3ng/ml interfere with the assay, which suggest that the assay mainly detect the free IL-22
Specifications
| Q9JJY9 | |
| 8 pg/mL | |
| ELISA Kit | |
| Mouse | |
| Colorimetric Microplate Reader | |
| GMCSF | |
| HRP | |
| Serum, 100 μL; Supernatant, 100 μL | |
| Interleukin-22 | |
| 1 hr. 45 min. |
| 8-1,000 pg/mL | |
| Biotin | |
| Serum, Supernatant | |
| ELISA | |
| 50929 | |
| Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate:Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86) | |
| RUO | |
| 2°C to 8°C | |
| Mouse | |
| 24 hr. 30 min. |
For Research Use Only