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Invitrogen™ IL-23 Mouse Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of mouse IL-23 protein levels from samples including serum, and cell culture supernatants.
2525.00 SEK - 6390.00 SEK
Specifications
| Accession Number | Q9EQ14 |
|---|---|
| Assay Range | 8-1,000 pg/mL |
| Assay Sensitivity | 8 pg/mL |
| Conjugate | Biotin |
| Product Type | ELISA Kit |
Alert:
Contains Azide
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
15581077
|
Invitrogen™
88-7230-86 |
10 x 96 Tests |
6390.00 SEK
Pack of 10 |
Estimated Shipment: 30-04-2024
Log in to see stock. |
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|
15571077
|
Invitrogen™
88-7230-22 |
2 x 96 Tests |
2525.00 SEK
Pack of 2 |
Estimated Shipment: 28-05-2024
Log in to see stock. |
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Description
The Mouse Interleukin 23 (IL-23) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-23. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Mouse IL-23 (p19/p40) ELISA Ready-SET-Go! 'IS' ELISA Set (Improved sensitivity assay with 5B2 capture antibody) with high affinity binding ELISA plates contains the necessary reagents, buffers and diluents for performing quantitative, enzyme-linked immunosorbent assays (ELISA)- The 5B2 antibody in combination with the C17.8 antibody yields an improved IL-23 sandwich ELISA with greater sensitivity than was achievable with previous assays
- For measurement of total p40 protein levels, the Mouse IL-12/23 Total p40 ELISA Ready-SET-Go! Is available (88-7120)
Specifications
| Q9EQ14 | |
| 8 pg/mL | |
| ELISA Kit | |
| Mouse | |
| Colorimetric Microplate Reader | |
| IL-21,IL-22,IL-D110,IL-TIF,ILTIF,TIFIL-23,TIFa,zcyto18 | |
| HRP | |
| Serum, 100 μL; Supernatant, 100 μL | |
| Interleukin-23, IL23, p40, p19 | |
| 1 hr. 45 min. |
| 8-1,000 pg/mL | |
| Biotin | |
| Serum, Supernatant | |
| ELISA | |
| 83430 | |
| Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 flat-bottom (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86) | |
| RUO | |
| 2°C to 8°C | |
| Mouse | |
| 24 hr. 30 min. |
For Research Use Only