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Invitrogen™ CD40L (Soluble) Mouse ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ BMS6010TEN
Additional Details : Weight : 0.23750kg
Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
Streptavidin-HRP
Mouse sCD40L Standard lyophilized, 40ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
Description
The Mouse CD40 Ligand (Ms CD40L) ELISA quantitates Ms CD40L in mouse serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CD40L. Principle of the method The Mouse CD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.Specifications
| 0.14ng/mL | |
| Cell Culture Supernatant, Serum | |
| ELISA | |
| 50 μL | |
| sCD154 (sCD40 Ligand) |
| Biotin | |
| Mouse | |
| 10 x 96 Tests | |
| TRAP, HIGM 1, T cell antigen Gp39, CD154 |
For Research Use Only