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Invitrogen™ eBioscience™ Mouse sCD40L Platinum ELISA Kit

For the quantitative detection of mouse sCD40L.

Brand:  Invitrogen™ BMS6010

Additional Details : Weight : 0.23750kg

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Product Code. 15501977

  • 4930.00 SEK / 96 tests


EU Stock 5
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
Streptavidin-HRP
Mouse sCD40L Standard lyophilized, 40ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD154 stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD154 has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Further, CD154 also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2. A defect in CD154 gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. CD154 is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. CD154 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD154 also appears to be involved in the transduction of regulatory signals for cellular functions such as B cell proliferation and differentiation.
Specifications

Specifications

P27548
Biotin
21947
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 tests
0.14 ng/mL
50μL
Mouse
1 hr 20 min
Mouse
TRAP, HIGM 1, T cell antigen Gp39, CD154
6.5%
0.31-20.0 ng/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Serum, Supernatants
Serum (50μL), Supernatants (50μL)
2-8°C
3 hr 10 min
Cd40lg
11.1%
HRP
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