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Invitrogen™ Th1/Th2 Mouse Uncoated ELISA Kit

Uncoated ELISA Kit

Brand:  Invitrogen™ 88-7711-44

Additional Details : Weight : 0.47500kg

Product Code. 15521177

  • 4355.00 SEK / 1 set


Estimated Shipment date
from Supplier 17-06-2020
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Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

T helper type 1 (Th1) cells and T helper type 2 (Th2) cells are both a lineage of CD4 effector T cells. Th1 and Th2 are involved in promoting cell-mediated immunity. Th1 cells secrete IFN-gamma, IL-2, IL-10, and TNF-alpha/beta, which are required for host defense against intracellular viral and bacterial pathogens. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-15, which are required for humoral immunity and play an important role in coordinating the immune response to larger extracellular pathogens.
Specifications

Specifications

P01580,P04351,P07750,P18893
HRP
Biotin
15978, 16153, 16183, 16189
ELISA Kit
IFN-gamma: 15 pg/mL; IL-2: 2 pg/mL; IL-4: 4 pg/mL; IL-10: 30 pg/mL
2°C to 8°C
24 hr. 30 min.
IFG,IFI
96 Tests
Supernatant
Colorimetric Microplate Reader
IFN-gamma: 15-2,000 pg/mL; IL-2: 2-200 pg/mL; IL-4: 4-500 pg/mL; IL-10: 30-4,000 pg/mL
ELISA
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
RUO
Plasma, 100 μL|Serum, 100 μL|Supernatant, 100 μL
1 hr. 45 min.
Mouse
IFNg, IL-10, IL-2, IL-4
Mouse
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