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Invitrogen™ TSLP Mouse Uncoated ELISA Kit with Plates

Engineered for the accurate and precise measurement of mouse TSLP in serum, and tissue culture supernatant samples.

Brand:  Invitrogen™ 88-7490-86

3486.84 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.47500kg

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Product Code. 15561157

  • 5230.00 SEK / Pack of 10
Estimated Shipment: 08-05-2024
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Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X Concentrated
Detection Enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for the dilution of antibodies and standards

Description

Description

The Mouse TSLP Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse TSLP. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
Specifications

Specifications

Q9JIE6
16 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
IL-2,TCGF,lymphokine
HRP
RUO
2°C to 8°C
Mouse
24 hr. 30 min.
16-2,000 pg/mL
Biotin
Serum, Supernatant
ELISA
53603
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards, 96-Well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
10 x 96 Tests
Serum, 100 μL; Supernatant, 100 μL
Thymic Stromal-Derived Lymphopoietin
1 hr. 45 min.
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