missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ VEGFB Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMVEGFB

3713.51 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.00400kg

Product Code. 15475353

  • 5570.00 SEK / 1 set
Estimated Shipment: 16-04-2024
to see stock.
Add to basket

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Mouse VEGF-B ELISA quantitates Ms VEGF-B in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms VEGF-B. Principle of the method The Mouse VEGF-B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.
Specifications

Specifications

P49766
0.6 ng/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
22340
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Angiogenesis
2°C to 8°C
Mouse
4 hr. 45 min.
0.655-160 ng/mL
0.6 ng/ml to 160 ng/ml
Biotin
Chemokine & Receptors
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS1, Adiponectin, ANG-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin, IFN-gamma R1, IL-17B, IL-17B R, IL-22, MIP-1 beta, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGF beta-1, TRANCE, TremL1, TWEAK, VEGF-R2.
Mouse
Colorimetric Microplate Reader
VEGF-B,Vrf
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
VEGF-B, Vrf
1 hr. 20 min.
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers