missing translation for 'onlineSavingsMsg'
Läs mer
  1. Home
  2. Products
  3. Proteinanalysreagenser
  4. Kompetenta celler
  5. 31781242

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli Cells, 96-well plate

Produktkod. 31781242
Change view
Klicka för att se tillgängliga alternativ
Kvantitet:
1 x 96 Well Plate (20 μL/Well)
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Produktkod. Kvantitet
31781242 1 x 96 Well Plate (20 μL/Well)
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Produktkod. 31781242 Leverantör Invitrogen™ Leverantörsnummer A40007058

Vänligen för att beställa denna produkten. Behöver du ett Webbkonto? Registrera ditt konto idag!

Beskrivning

MultiShot TOP10 Chemically Competent E. coli Cells provide high-efficiency transformation for routine cloning in an automation-ready 96-well format. Ideal for stable propagation of high-copy plasmids and blue/white screening in high-throughput workflows.

Invitrogen MultiShot TOP10 Chemically Competent E. coli Cells are derived from DH10B cells and are one of the most popular laboratory strains. Provided in a clear skirted 96-well microtiter plate to simplify high-throughput workflows and provide transformation efficiencies of >1 x 108 cfu/μg control plasmid DNA, Multishot TOP10 cells are a versatile strain that can be used in multiple routine cloning applications.

Features of MultiShot TOP10 competent cells include:

  • TOP10 strain ideal for high-efficiency cloning and plasmid propagation
  • Microtiter plate format design convenient for automated high-throughput cloning
  • mcrA mutation that supports efficient transfection of methylated DNA
  • lacZΔM15 allele that permits rapid blue/white screening of transfectants
  • endA1 mutation that helps reduce endonuclease activity and improve plasmid DNA yields
  • recA1 mutation helps reduce unwanted recombination

MultiShot TOP10 competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.

Genotype

F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

TRUSTED_SUSTAINABILITY

Specifikationer

Produkttyp Chemically Competent Cells
Inkluderar pUC19 DNA, S.O.C media
Transformationseffektivitetsnivå ≥1.0 x 108
Frakt skick Dry Ice
Art E. coli
Formatera 96-well Plate
Produktlinje MultiShot
För användning med (applikation) Cloning
Kvantitet 1 x 96 Well Plate (20 μL/Well)

For Research Use Only.

Produkttitel
Välj ett problem

Genom att klicka på Skicka bekräftar du att du kan bli kontaktad av Fisher Scientific angående feedbacken du har lämnat i detta formulär. Vi kommer inte att dela din information för andra ändamål. All kontaktinformation som tillhandahålls ska också underhållas i enlighet med vår Sekretesspolicy.