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MYLK (MLCK) Recombinant Human Protein
MYLK (MLCK) Recombinant Human Protein
Brand: Invitrogen PV4339
Additional Details : Weight : 0.00945kg
Description
MYLK (MLCK) is a serine⁄threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction).Specifications
NP_444253 | |
Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
67.3kDa | |
10μg | |
For research use only. Not for use in diagnostic procedures | |
MYLK (MLCK) | |
MYLK (MLCK) was pre-diluted in enzyme dilution buffer and assayed in 10mM HEPES (pH 7.5), 10mM MgCl2, 1mM Na3VO4, 10μg/mL calmodulin, 0.5mM CaCl2, 2.5mM DTT, 200μM ATP, 200μg/mL ZIPtide peptide substrate (KKLNRTLSFAEPG) and trace [32P]-γ-ATP for 10 minutes at 30°C | |
Amino acids 1425-1771, aa 1428-1771 | |
76nmoles of phosphate transferred to ZIPtide peptide substrate (KKLNRTLSFAEPG) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 8.33μg/mL | |
GST Tag | |
Kinase and inhibitors | |
80% by SDS-PAGE |
0.39mg/mL | |
4638 | |
MYLK (MLCK) Recombinant Human Protein | |
–80°C | |
1 Each | |
MYLK (MLCK) Recombinant Human Protein | |
MLCK | |
MYLK (MLCK) Recombinant Human Protein | |
Human | |
Baculovirus | |
Serine/threonine kinase |
For Research Use Only. Not for use in diagnostic procedures.