Nestin, clone: Rat-401 (Rat401 (4D4)), eBioscience™

Description

Description: The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells. Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells. Nestin expression is also present in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells. Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers. Upon differentiation, Nestin-containing filaments are replaced by cell type specific intermediate filament proteins, such as GFAP.Applications Reported: This Rat-401 (Rat401 (4D4)) antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This Rat-401 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells and by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.