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Invitrogen™ NFkB p65 (Total/Phospho) Human InstantOne™ ELISA Kit
InstantOne ELISA
Brand: Invitrogen™ 85-86083-11
4793.57 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Additional Details : Weight : 0.23750kg
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Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
NFkB (Total) Capture Antibody Reagent
phospho-NFkB (Ser536) Capture Antibody Reagent
NFkB (Total) Detection Antibody Reagent
phospho-NFkB (Ser536) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate Seal
Description
The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human NFkB p65 in cell lysates. Detection of mouse and rat NFkB is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
NF-kappaB (Nuclear Factor kappa B) is a nuclear transcription factor found in all cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, and bacterial or viral antigens. NF-kappaB plays a key role in regulating the immune response to infection. Consistent with this role, incorrect regulation of NF-kappaB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection and improper immune development. There are five members in the NF-kappaB family: NF-kappaB1, NF-kappaB2, RelA (also named p65), RelB, and c-Rel. The most common form of NF-kappaB is the p50/RelA heterodimer, although other forms of NF-kappaB dimers, such as p50/p50, p52/p52, p52/RelA, p50/c-Rel, c-Rel/c-Rel, p52/RelB, and p50/RelB, have also been identified in some types of cells. The primary role of NF-kappaB is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. The activity of NF-kappaB is tightly regulated by interaction with inhibitory IkappaB proteins.Specifications
Q04206 | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
p65,NFKB3 | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
1 hr. 10 min. |
Biotin | |
Cell Lysates | |
ELISA | |
5970 | |
Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal | |
96 Tests | |
Cell Lysate,20 μL | |
RELA, NFKB3 | |
1 hr. 20 min. |
For Research Use Only