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NK1.1, FITC, clone: PK136, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-5941-81

Additional Details : Weight : 0.25000kg

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Product Code. 15258299

  • 668.00 SEK / 50µg


EU Stock 3
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Description

Description

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 14-5971 should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: PK136 has been reported for use in flow cytometric analysis.Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

NK1.1
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P27814
Klrb1d, Ly55b, Ly55d, MGC163757, MGC163759, NKR-P1B, NKR-P1D, Nkrp1-b, Nkrp1d
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
PK136
FITC
Klrb1c
Liquid
Klrb1c
IgG2a, kappa, kappa
50μg
4° C, store in dark, DO NOT FREEZE!
Primary
17059
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