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Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
POA
Specifications
| Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
|---|---|
| Membrane Compatibility | PVDF, Nitrocellulose |
| Product Line | Invitrogen™ |
| For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
| Run Time | 10 minutes |
| Product Code | Brand | No. of Reactions | Gel Compatibility | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Gel Compatibility | Price | Quantity & Availability | ||||
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16351358
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Invitrogen™
A44717 |
A standard kit will label 10 mini gels or 10 mini gel-sized membranes | Mini Precast Protein Gels, Midi Precast Protein Gels |
POA
10 reactions |
N/A | Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
This item requires a price update prior to being ordered. Request a Price for this item here. |
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16341358
|
Invitrogen™
A44449 |
A standard kit will label 40 mini gels or 40 mini gel-sized membranes | Any Gel Type and Gel Chemistry |
POA
40 reactions |
N/A | Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
This item requires a price update prior to being ordered. Request a Price for this item here. |
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Description
- Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
- Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
- Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
- Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
- Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
- Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.
Specifications
| The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins | |
| Invitrogen™ | |
| 10 minutes | |
| Protein Labeling Reagent |
| PVDF, Nitrocellulose | |
| Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager | |
| Dry Ice | |
| Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
For Research Use Only. Not for use in diagnostic procedures.