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Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Brand:  Thermo Scientific™ 37555

Additional Details : Weight : 0.80000kg

Product Code. 11869320

  • 1462.00 SEK / 375mL
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Description

Description

Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
Specifications

Specifications

375 mL
North2South Hybridization Stringency Wash Buffer (2X)
Yields 60mM sodium citrate, 600mM sodium chloride, 0.2% SDS, pH 7.9 when diluted 1:1 in water
Non-radioactive Southern and Northern blotting
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