NOTCH1, PE, clone: 22E6, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5765-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells. Lower Notch1 expression levels can be found on B cells and monocytes. Studies show that some subsets of T-cell acute lymphoblastic leukemia (T-ALL) arise due to Notch1 chromosomal translocation with TCRbeta, which results in the expression of constitutively active Notch1. This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation.Applications Reported: This 22E5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 22E5 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|9930111A19Rik, Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila), lin-12, major type A protein, Mis6, Motch A, mT14, N1, neurogenic locus notch homolog protein 1, Notch gene homolog 1, Notch homolog 1, translocation-associated, p300, RP23-306D20.12-001, Tan1, translocation-associated notch protein TAN-1, transmembrane receptor Notch1|
|IgG2a, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.