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Invitrogen™ Dynabeads™ M-280 Sheep Anti-Mouse IgG Product Code.: 10077512

Invitrogen™ Dynabeads™ M-280 Sheep Anti-Mouse IgG

Product Code. 10077512
10 mL, 10mL
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Quantity:
2 mL
10 mL
Unit Size:
10mL
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10077512

Brand: Invitrogen™ 11202D

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Includes

2mL Dynabeads M-280 Sheep anti-Mouse IgG (10mg beads/mL)

Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-ÎĽm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface.

Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-ÎĽm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface. These beads are intended for use as a solid support for simple and efficient binding of mouse IgGs and their target proteins. The uniform, monodisperse, and non-porous Dynabeads magnetic beads make them an ideal choice for applications such as binding immunoglobulins, protein purification, sandwich immunoassays, immunoprecipitation (IP), Co-immunoprecipitation (Co-IP), and isolation of cells and microorganisms. The polyclonal sheep antibody binds mouse IgG1, IgG2a, and IgG2b, and, with a lower reactivity, mouse IgG3 and IgM. Human cross-reactivity is minimal.

Benefits of Dynabeads M-280 Sheep anti-Mouse IgG beads include:

  • Speed—isolate protein in less than 40 minutes
  • Selectivity—binds most mouse IgG subclasses, very low non-specific binding
  • Flexibility—no columns, centrifugations, or time-consuming pre-clearing required
  • Automation ready—automation protocols available with the KingFisher Sample Purification instruments

Great signal to noise ratio

Dynabeads M-280 Sheep anti-Mouse IgG beads covalently couple the secondary sheep antibodies to a hydrophobic surface, which means the non-specific hydrophobic interactions are reduced to a minimum. Since this interaction is the main driver of unwanted protein binding, Dynabeads magnetic beads help ensure a great signal-to-noise ratio. This Dynabeads magnetic bead platform binds any mouse IgG antibody in a solution and can therefore become a powerful tool in affinity purification.

Dynabeads immunoprecipitation protocol is fast and easy

Add the target specific primary mouse IgG antibody directly to the sample (indirect technique) or first pre-coat the target antibody onto the Dynabeads M-280 Sheep Anti-Mouse IgG beads (direct technique). Regardless of which technique is used, when the Dynabeads M-280 Sheep anti-Mouse IgG beads are mixed with the sample, they bind to the target. The manual IP protocol is simple and can be performed in less than 40 minutes with 3 easy bind-incubate-elute steps. The procedure does not require any pre-clearing due to the low non-specific binding and uses very little antibody while still obtaining a high IP yield, thus saving both time and cost per IP.

The antibody-coated beads can be used in a variety of IP applications such as Co-IP, chromatin IP (ChIP/ChIP sequencing), RNA IP (RIP, RIP sequencing), small-scale IgG purification, and protein purification. The isolated pure target protein can be used in downstream western blot, mass spectrometry analysis, sequencing, etc.

Automation-ready for use with KingFisher purification systems

The 2.8-ÎĽm magnetic beads are ideal for high-throughput enrichment and can be automated using any of our KingFisher sample purification systems.

Commercial supply

Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Sheep anti-Mouse IgG beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:

  • See our immunoprecipitation selection table, data, and references
  • Dynabeads magnetic beads selection guide
  • See magnets for Dynabeads magnetic beads separations
  • KingFisher automation protocols
  • Watch a video about the KingFisher Flex instrument

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 mg/mL
Description Polyclonal sheep anti-mouse IgG covalently bound to Dynabeads
Material Polystyrene
Content And Storage Dynabeads M-280 Sheep Anti-Mouse IgG is supplied in PBS, pH 7.4 w/0.1% BSA, 0.02% NaN3 Store at 2°C to 8°C.
Diameter (Metric) 2.8 ÎĽm
For Use With (Equipment) KingFisher™ Sample Purification Systems, DynaMag™ Magnets
High-throughput Compatibility High-throughput Compatible
Shipping Condition Ambient temperature
Reactivity Binds mouse IgG1, IgG2a and IgG2b. Low reactivity towards mouse IgG3 and IgM.
Quantity 10 mL
For Use With (Application) Protein Purification
Target Proteins
Type Antibody Coated Bead
Ligand Type Antibody
Species Sheep
Format Beads in suspension
Color Brown
Certifications/Compliance ISO9001 and ISO13485
Regulatory Status For Research Use Only
Uniformity Monosized 2.8 ÎĽm (CV <5%)
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number2760906 Certificate TypeCertificate of Analysis Date29/01/2025 Product Code11202D
Lot Number2820407 Certificate TypeCertificate of Analysis Date29/01/2025 Product Code11202D
Lot Number2827664 Certificate TypeCertificate of Analysis Date28/01/2025 Product Code11202D
Lot Number2860961 Certificate TypeCertificate of Analysis Date28/01/2025 Product Code11202D
Lot Number2907520 Certificate TypeCertificate of Analysis Date27/01/2025 Product Code11202D

For Research Use Only. Not for use in diagnostic procedures.

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