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Invitrogen™ IL-4 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHC0041

4146.87 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.50000kg

Product Code. 10521475

  • 6220.00 SEK / 96 tests
Estimated Shipment: 11-04-2024
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For Research Use Only. All usage must comply with product instructions.


Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Human Interleukin 4 (Hu IL-4) ELISA quantitates Hu IL-4 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-4. Principle of the method The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

P05112
<2 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
BCGF-1,BCGF1,BSF-1,BSF1,IL-4
3%
HRP
96 Tests
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Human
3 hr.
7.8-500 pg/mL
Biotin
Plasma, Serum, Supernatant
Colorimetric
3565
4.5%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
<2pg/mL
RUO
2°C to 8°C
1 hr. 20 min.
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For Research Use Only. Not for use in diagnostic procedures.