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  5. Invitrogen™ Humant IL-8 ELISA Utrustning 

Invitrogen™ Humant IL-8 ELISA Utrustning

Product Code. 10232593
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Kvantitet:
2 x 96 tester
5 x 96 tester
96 tester
Unit Size:
192 tester
96 tester
Paket med 5
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10232593 2 x 96 tester 192 tester
10786703 96 tester 96 tester
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For Research Use Only. All usage must comply with product instructions.
Product Code. 10232593 Supplier Invitrogen™ Supplier No. KHC0082
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Beräknad leverans: 20-08-2026
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9471.50 SEK valid until 2026-12-31
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For Research Use Only. All usage must comply with product instructions.

Description

Includes

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Human Interleukin 8 (Hu IL-8) ELISA kvantifierar Hu IL-8 i humant serum, plasma, buffrad lösning eller cellodlingsmedium. Analysen kommer uteslutande att känna igen både naturligt och rekombinant Hu IL-8. Metodens princip The Human IL-8 solid-phase sandwich ELISA (enzymkopplad immunosorbentanalys) är utformad för att mäta mängden av målet som är bundet mellan ett matchat antikroppspar. En målspecifik antikropp har förbelagts i brunnarna på den medföljande mikroplattan. Prover, standarder eller kontroller läggs sedan till dessa brunnar och binder till den immobiliserade (infångande) antikroppen. Sandwichen bildas genom tillsats av den andra (detektor) antikroppen, en substratlösning tillsätts som reagerar med enzym-antikropp-mål-komplexet för att producera en mätbar signal. Intensiteten av denna signal är direkt proportionell mot koncentrationen av mål som finns i det ursprungliga provet. Rigorös validering Varje tillverkad parti av detta ELISA kit är kvalitetstestat för kriterier som känslighet, specificitet, precision och lot-to-lot-konsistens. Se manualen för mer information om validering.

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

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Specifications

Tillträdesnummer P10145
Analysintervall 15,6 till 1000 pg/ml
Analyskänslighet <5 pg/mL
Innehåll och lagring Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Konjugera HRP
Immunoassay Kit Format Sandwich ELISA Kit
Produkttyp ELISA
Proteinfamilj Chemokines & Receptors
Provtyp Plasma, Serum, Supernatant
Målarter Människa
För användning med (applikation) Kolorimetrisk
För användning med (utrustning) Kolorimetrisk mikroplattläsare
Gene Alias (Ala-IL-8)77; (Ser-IL-8)72; Alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; AMCF-I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; CXCL8; cytokine 8; emoctakin; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; GCP-1; Granulocyte chemotactic protein 1; IL8; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin8; interleukin-8; interleuklin-8 precursor; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil activating peptide 1; neutrophil and T-lymphocyte chemotactic and activating factor; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Permeability factor 1; PF1; Protein 3-10C; RP11-537A6.8; RPF1; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1(Ala-IL-8)77; (Ser-IL-8)72; Alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; AMCF-I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; CXCL8; cytokine 8; emoctakin; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; GCP-1; Granulocyte chemotactic protein 1; IL8; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin8; interleukin-8; interleuklin-8 precursor; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil activating peptide 1; neutrophil and T-lymphocyte chemotactic and activating factor; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Permeability factor 1; PF1; Protein 3-10C; RP11-537A6.8; RPF1; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1
Gen-ID (Entrez) 3576
Gensymbol GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Interassay CV 6.1%
Intraassay CV 3.9%
Kit Innehåll Förbelagd 96 brunnars platta, Standard, Standard utspädningsbuffert, Biotinylerad detektionsantikropp, Streptavidin-HRP, HRP-spädningsmedel, Tvättbuffert, Kromogen, Stoppa lösning, Självhäftande plåtskydd
Etikett eller färgämne Biotin
Precision <5 pg/mL
Kvantitet 2 x 96 tester
Regulatorisk status RUO
Provvolym Plasma 50μ L, Serum 50μ L, Supernatant 50μ L
Frakt skick Wet Ice
Förvaringskrav 2 °C till 8 °C
Mål IL-8
Testtid 1 tim 20 min
Total analystid 2 timmar 30 minuter
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