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Invitrogen™ IL-6 Human Matched Antibody Pair

Produktkod. 10353493
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10353493 10 x 96 Tests
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For Research Use Only. All usage must comply with product instructions.
Produktkod. 10353493 Leverantör Invitrogen™ Leverantörsnummer CHC1263

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4681.80 SEK valid until 2026-03-27
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For Research Use Only. All usage must comply with product instructions.

Beskrivning

Innehåller

Antibody Pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.

Kräver

Buffer reagents required to complete reaction available separately (Cat. No. CNB0011).

Matched Antibody Pair

The Human IL-6 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-6. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Beställningsinfo

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY

Specifikationer

Konjugera HRP
Genaccessionsnr. P05231
Gene Alias BSF2, HGF, HSF, IFNB2, IL-6
Gensymboler IL6
Kvantitet 10 x 96 Tests
Gen-ID (Entrez) 3569
Inkluderar Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Målarter Human
Innehåll och lagring 2°C to 8°C
Produkttyp ELISA Kit
För användning med (utrustning) Colorimetric Microplate Reader
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For Research Use Only. Not for use in diagnostic procedures.

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